Cardiospermum halicacabum suppresses the production of TNF-alpha and Nitric oxide by Human Peripheral Blood Mononuclear cells

Authors

  • K.C. Venkatesh Babu Author
  • S. Krishnakumari Author

DOI:

https://doi.org/10.4314/

Keywords:

Cardiospermum halicacabum, nitric oxide, TNF a

Abstract

Cardiospermum halicacabum is an Asian and African herb known to 
possess anti-inflammatory activities. The inhibitory effects of the ethanolic 
fraction of Cardiospermum halicacabum leaves extract,(EFC) on the 
production of pro-inflammatory mediators, nitric oxide(NO) and tumor 
necrosis factor-alpha (TNF a) were studied in a lipopolysacchride (LPS) 
activated human peripheral blood mononuclear cells. TNF a production 
was measured based on the selective sensitivity of TNF a for L929 
tumorigenic murine cells. Similarly LPS stimulated macrophages cell line 
RAW 264.7 was used for NO inhibition studies. EFC potently inhibited 
TNF a (5-40µg/mL) with IC50=17 µg/mL, and NO (20-100 µg/mL) with 
IC50 value being 90µg/mL.The viability of cells at all the concentration 
was unaffected as determined by the MTS cytotoxicity
assay.C.halicacabum thus exhibits the anti-inflammatory properties, 
justifying its use in rheumatoid arthritis treatment

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Published

2024-09-15

Issue

Vol. 9 No. 2 (2006): May Issue

Section

Original Article

License

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

Cardiospermum halicacabum suppresses the production of TNF-alpha and Nitric oxide by Human Peripheral Blood Mononuclear cells. (2024). African Journal of Biomedical Research, 9(2), 95-99. https://doi.org/10.4314/