In Vitro Regeneration of Gloriosa Superba L. Using Shoot Tip and Nodal Segment Explants

Abstract

Culture conditions were developed for in vitro regeneration of Gloriosa superba L. using shoot tip and nodal segment explants. In case of both explants, the best shoot induction was observed on MS+1.5 mg/l BAP+0.5 mg/l NAA. Nodal explants showed more effective forshoot proliferation than shoot tip explants. Among two types of explants, nodal segment explants produced the highest number of shoots (8±0.12) when they were cultured on above mentioned medium. Shoot tip explants also produced multiple shoots, but their performance was not as good as nodal explants. Addition of 10% coconut water (CW) to the above mentioned medium enhanced the number of shoots per culture and incorporation of 100mg/l urea to the medium increased the length of shoots. Best rooting was obtained from shoots cultured on half-strength of MS fortified with 1.0 mg/l IBA+ 0.5 mg/l NAA. Within four weeks of transfer to the rooting medium, 80% microcuttings produced 8-10 roots. The regenerated plantlets were successfully transferred to potted soil, where survival rate was 80%.