In-Vitro Antibacterial Activity of Three Traditionally Used Medicinal Plants Against Multidrug Resistant Pseudomonas Aeruginosa

Abstract

The current study involves the in-vitro screening of the antibacterial activities of three traditionally used therapeutic plants viz. Hippophae salicifolia, Phyllanthus emblica and Swertia chirata against multidrug resistant Pseudomonas aeruginosa. The screening of the antibacterial effect of the plant extracts was done by agar well diffusion assay and Minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MBC) methods. The diameter of the zone of inhibition (ZOI) was measured. Among the three extract used, highest antibacterial activity was recorded with petroleum ether extract of Hippophae salicifolia. Initially,the phytochemical examination of the plant extract from different plant parts  revealed the presence of active compounds sterols, phenols, saponins, alkaloids and flavonoids. The results obtained in this analysis demonstrated that the antibacterial activity of Hippophae salicifolia with petroleum ether extract and phenolic content on MDR Pseudomonas aeruginosa is satisfactory and this is due to the presence of secondary metabolites or active compounds .In comparison to  the antibacterial activities of three extracts it was however elucidated that Hippophae salicifolia was the most effective and powerful plant against the tested multidrug resistant Pseudomonas aeruginosa.