Antinociceptive and Anti-inflammatory properties of a polyherbal extract of Plumbago zeylinica and Capsicum frutescens in rodents

Abstract

The aim of the present study is to evaluate the anti-nociceptive and anti-inflammatory properties of an extract of a polyherbal 
mixture containing the leaves and roots of Plumbago zeylinica and fruits of Capsicum frutescens. Acute toxicity effect of WAP 
was evaluated in mice. The antinociceptive and anti-inflammatory activities of WAP (100–400 mg/kg) were investigated in hot 
plate, acetic acid-induced writhing, carrageenan-induced paw oedema and air pouch models. No death was recorded when WAP 
was administered at a dose of 2000 mg/kg. Oral treatment with WAP elicited inhibitory activity in hot plate test. Abdominal 
writhing was significantly reduced by WAP at 100, 200 and 400 mg/kg (54.5 ± 1.6, 20.0 ± 7.4, 38.4 ± 4.8) as compared with the
control group (71.0 ± 2.6). Carrageenan-induced oedema formation was significantly (p < 0.05) reduced at WAP (100, 200, and 
400 mg/kg) by 23.5, 27.3 and 31.4%, respectively, and by reference to indomethacin (10 mg/kg, 31.2%). WAP (200 and 400 
mg/kg) significantly reduced exudate volume (37.2 and 44.1%), protein (42.0 and 54.1%), total leucocytes (59.1 and 60.2) and 
neutrophils count (57.3 and 58.0%) in the carrageenan-induced air pouch in rats. Similarly, WAP treated animals showed reduced 
nitrites and malondialdehyde levels and increased glutathione levels. Furthermore, WAP (400 mg/kg) nether affected locomotory 
activity nor induced gastric lesion in mice. The results of this study revealed that extract of a polyherbal mixture containing the 
leaves and roots of Plumbago zeylinica and fruits of Capsicum frutescens possesses anti-noiceptive and anti-inflammatory 
activity