Presence of GFSKLYFamide-like Neuropeptide in the Tentacles, Body Wall and Tube Foot of Holothuria scabra: Immunohistochemical Evidence

Authors

  • A. Ajayi Author
  • N.O. Amedu Author
  • B. Withyachumnarnkul Author

DOI:

https://doi.org/10.4314/

Keywords:

GFSKLYFamide, immunohistochemistry, Holothuria scabra, tube foot, tentacles, body wall

Abstract

The neuropeptide GFSKLYFamide is a heptapeptide first isolated from the sea cucumber, Holothuria glaberimma. The holothurian 
body wall, tentacles and tube foot are important organs for locomotion, feeding and maintenance of homeostasis. This study was 
aimed at determining the localization of GFSKLYFamide in the tentacles, body wall and tube foot of Holothuria scabra. Ten male 
H. scabra weighing between 62g and 175g were used for this study. Using GFSKLYFamide polyclonal antibody and Alexa 488-
conjugated goat anti-rabbit IgG as primary and secondary antibodies, respectively, indirect immunofluorescence method with
confocal microscope imaging were used to localise GFSKLYFamide immunoreactivity in the tentacles, body wall and tube foot of
H. scabra. The results indicate the presence of GFSKLYFamide-like neuropeptide in the epithelial layers of the tentacles, body wall 
and tube foot. GFSKLYFamide-like immunoreactivity was also detected within the connective tissue layer of the tentacles and tube 
foot.

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Published

2024-07-09

Issue

Vol. 23 No. 1 (2020): January Issue

Section

Original Article

License

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

Presence of GFSKLYFamide-like Neuropeptide in the Tentacles, Body Wall and Tube Foot of Holothuria scabra: Immunohistochemical Evidence. (2024). African Journal of Biomedical Research, 23(1), 25-28. https://doi.org/10.4314/

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