THERMOSTABILITY OF RECONSTITUTED NEWCASTLE DISEASE VIRUS STRAINS AT 360 C TEMPERATURE

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DOI:

https://doi.org/10.4314/

Keywords:

Haemagglutination, Thermostability, Newcastle Disease Virus, Reconstituted and Strain

Abstract

Haemagglutination (HA) test was employed to determine the stability of HA titers of reconstituted form of 
Hitchner – B1 (B1), LaSota (L) and Komarov (K) strains of Newcastle Disease Vaccine (NDV) at 360
c. The 
temperature treatment method was through incubation (in water bath) of the reconstituted vaccines at selected 
temperature and sequential sampling of each vaccine vial for the determination of pre – and post – temperature 
exposure HA titers. Thus, on the basis of a two-step (2log2) decline in titer as evidence of loss of stability of HA 
titers (LST), the LST therefore, occurred at 50th, 24th and 95th hour for BI, L and K strains, respectively, post – 
temperature exposure. The data, therefore, showed that the NDV – K strain was the most stable at the test 
temperature. It is believed that the findings will enhance the understanding of the potential of this strain in the 
developed and application of a thermostable MDV target on village poultry. In rural settings. 

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Published

2024-09-15

Issue

Vol. 5 No. 1&2 (2002): Combined Issue

Section

Original Article

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This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

THERMOSTABILITY OF RECONSTITUTED NEWCASTLE DISEASE VIRUS STRAINS AT 360 C TEMPERATURE. (2024). African Journal of Biomedical Research, 5(1&2), 87-89. https://doi.org/10.4314/